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. 2021 Jan 5;8:572356. doi: 10.3389/fbioe.2020.572356

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Copyright © 2021 Anderson-Baron, Liang, Kunze, Mulet-Sierra, Osswald, Ansari, Seikaly and Adesida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PTHrP does not affect Safranin-O staining in cocultured pellets with interaction index < 1 (i.e., Non-Responders). After 3 weeks of culture in chondrogenic media, pellets were fixed, embedded, and sectioned for histology. (A) The GAG per DNA contents of the monoculture and cocultured pellets associated with interaction index > 1. (B) The interaction index of cocultured pellets. (C–H) Safranin O staining of pellets formed from 250,000 cells and (I–N) safranin staining of pellets formed from 500,000 cells. The main and inset images are at 200x and 100x optical magnification, respectively. Scale bar are 100 μm. Statistical significance was determined using one-way ANOVA with Tukey’s HSD post hoc test (three donors in total; two donors in duplicates at 250,000 cells per pellet and one donor in duplicates at 500,000 cells per pellet; n = 6; ^∗∗∗p < 0.001) (n = 6; p > 0.05).