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. 2021 Jan 5;8:572356. doi: 10.3389/fbioe.2020.572356

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Copyright © 2021 Anderson-Baron, Liang, Kunze, Mulet-Sierra, Osswald, Ansari, Seikaly and Adesida.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PTHrP affected the expression of COL10A1 without other markers of chondrocyte hypertrophy in cocultured pellets with interaction indices > 1 (i.e., Responders). Pellets were lyzed in TRIzol and the RNA was extracted. cDNA was synthesized and gene expression was analyzed by qPCR. The expression of chondrogenic genes: (A) ACAN, (B) COL2A1, (C) SOX9, (D) COL1A2, (E) MMP13, (F) COL10A1, (G) IHH, (H) ALPL, and (I) ID1 were examined. Expression of each gene was normalized to B2M, HMBS, and YWHAZ and expressed as the 2^{– Δ} ^Δ ^Ct value, shown on the y-axis. The x-axis indicates the sample and treatment. Statistical significance was determined using one-way ANOVA with Games-Howell post hoc test of unequal variances assumed (three donors in total; two donors in duplicates at 250,000 cells per pellet and one donor in duplicates at 500,000 cells per pellet; n = 6; *p < 0.05).