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. 2021 Jan 5;10:615534. doi: 10.3389/fonc.2020.615534

Figure 1.

Figure 1

Cloning of antihuman CD47 monoclonal antibodies. (A) Representative flow cytometry of 3T3-WT and 3T3-CD47 (GFP) cell line after staining with B6H12.2. (B) Representative flow cytometry of Jurkat-shCON (GFP) and Jurkat-shCD47 (GFP) cell line after staining with B6H12.2. (C) A pair of Jurkat-GFP cells were stained with B6H12.2 (red) and DAPI (blue) and observed under a two-photon confocal microscope. Scale bar, 100 μm. The experiment was performed three times with similar results. (D) A mix of Jurkat-WT cells and Jurkat-shCD47-GFP cells were incubated with hybridoma supernatant and analyzed with flow cytometry. Hybridomas without antibody (left), hybridoma producing nonspecific antibody (middle), or hybridoma producing specific antibody (right). (E) A pair of Jurkat-GFP cells were stained with selected hybridoma supernatant (red) and DAPI (blue) and observed under a two-photon confocal microscope. Scale bar, 100 μm. The experiment was performed three times with similar results.