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. 2021 Jan 18;11:1740. doi: 10.1038/s41598-021-81039-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Transfection of microglia with miR-467f, miR-466q, or exposure to s-EV^IFN-γ-MSC, inhibits Map3k8 and Mk2 expression. (a) Scheme of p38 MAPK signaling pathway showing that Map3k8 and Mk2 are specific targets of miR-467f and miR-466q, respectively. (b) Exposure of LPS-activated N9 cells to s-EV^IFN-γ-MSC induced a significantly downregulation of Map3k8 and Mk2 mRNA expression. ^###P < 0.001, untreated (N9) vs LPS-activated N9 cells (N9 + LPS); **P < 0.01, ***P < 0.001, N9 + LPS vs N9 + LPS exposed to s-EV^IFN-γ-MSC (N9 + LPS + s-EV^IFN-γ-MSC). (c) Downregulation of Map3k8 and Mk2 mRNA expression in LPS-activated N9 cells transfected with miR-467f and miR-466q confirm that they are target genes for these miRNAs. ^###P < 0.001, untreated (N9) vs LPS-activated N9 cells (N9 + LPS); *P < 0.05, **P < 0.01, N9 + LPS vs N9 + LPS transfected with specific miRNA (N9 + LPS + miRNA). (d) Primary microglia obtained from SOD1^G93A mice transfected with miR-467f and miR-466q showed a significant downregulation of the expression of genes involved in the p38 MAPK signaling pathway. *P < 0.05, ***P < 0.001, SOD1^G93A vs SOD1^G93A + miR467f or 466q. Data are presented as mean ± SEM of 3 independent experiments conducted in triplicates.