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. 2021 Jan 18;12(1):94. doi: 10.1038/s41419-020-03334-8

Fig. 6. The hsa_circ_0007456 regulated the expression of ICAM-1 by sponging miR-6852-3p.

Fig. 6

A–J After infection with hsa_circ_0007456-overexpressing or shRNA lentivirus or the control lentivirus in SMMCC-7721 or QGY-7703, the relative RNA levels of ICAM-1 were detected by RT-qPCR (A–D); the expression of ICAM-1 (CD107a) on the cell surface was detected by flow cytometry (E, F); and the relative protein levels of ICAM-1 were detected by western blotting (G–J). K, L SMMCC-7721 or QGY-7703 stably expressing the luciferase construct containing the wild-type (WT) or mutant (MT) ICAM-1 were respectively infected with hsa_circ_0007456-overexpressing or shRNA lentivirus or the negative control lentivirus (NC), and then the luciferase activity was examined. M–R After treatment with the indicated hsa_circ_0007456 or the control lentivirus plus miR-6852-3p mimic or negative control (NC) in SMMCC-7721 or QGY-7703, the mRNA levels (M, N) and the protein levels (O–R) of ICAM-1 were respectively detected by RT-qPCR and western blotting. Data are representative of three independent experiments and are presented as mean ± SD. **p < 0.01.