Fig. 4. wTBS with PKA Cα transiently increases γ via CP-AMPAR insertion.
a–b A wTBS in the presence of intracellular PKA Cα (300 U/mL) transiently increased γ (n = 17 neurons from 13 animals, mean ± SEM, F(1.931,27.04) = 52.89 for EPSCs and F(1.863,26.08) = 12.59 for γ, one-way repeated measures ANOVA followed by Bonferroni’s multiple comparisons test; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. baseline). EPSCs (a) and γ (b) were analyzed in 10-min bins. A single episode of TBS (at time marked by an arrow) was delivered to one input (filled symbols) with the second input (open symbols) serving as a control; base = baseline. c A representative current–variance plot for PKA Cα plus wTBS for baseline, the first 10 min (LTP10’) and the last 10 min of LTP (LTP30’). Sample traces were obtained from baseline and LTP10’. Scale bars: 10 pA and 10 ms. d–f Equivalent experiments in the presence of IEM-1460 (IEM, 30 µM; n = 16 neurons from 13 animals, F(1.095,13.14) = 25.66 for EPSCs and F(2.184,26.21) = 0.2547 for γ, one-way repeated measures ANOVA followed by Bonferroni’s multiple comparisons test; *p < 0.05, **p < 0.01 and ***p < 0.001 vs. baseline). g, h Quantification of the levels of LTP (t31 = 3.006, p = 0.0052, two-sided unpaired Student’s t test) (g) and (t31 = 3.544, p = 0.0013, two-sided unpaired Student’s t test) γ (h) measured during the 10 min after wTBS with cumulative distributions (right). n = 17 neurons from 13 animals (PKA Cα + wTBS) and 16 neurons from 13 animals (PKA Cα + wTBS + IEM). i, j Analysis of the relationships between γ and LTP for PKA Cα + wTBS (p = 0.0021, F(1,15) = 13.72, F-test) (i) and PKA Cα + wTBS + IEM (p = 0.9090, F(1,14) = 0.0136, F-test) (j). k, l Analysis of the relationships between γ and EPSC decay time for PKA Cα + wTBS (p = 0.0117, F(1,15) = 8.243, F-test) (k) and PKA Cα + wTBS + IEM (p = 0.3931, F(1,14) = 0.7764, F-test) (l). Data are presented as mean ± SEM. Source data are provided as a Source Data file.