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. 2021 Jan 18;12(1):97. doi: 10.1038/s41419-020-03340-w

Fig. 3. MEDAG triggers EMT in breast cancer cells.

Fig. 3

A, B Western blots of the EMT-related markers E-cadherin, N-cadherin, and Snail in MEDAGKD cells and MEDAGOE of MCF-7 and MDA-MB-468 cell lines. C Representative IHC staining images of MEDAG and EMT-related markers in breast cancer samples (magnification 200x). D, E Correlation analysis of protein expression between MEDAG and the EMT-related markers E-cadherin and N-cadherin in breast cancer samples by IHC. FI Correlation analysis of the mRNA level between MEDAG and the EMT-related markers E-cadherin, N-cadherin, Snail and Vimentin in breast cancer from TCGA database. J, K MEDAG was overexpressed in MEDAGKD cells, and the EMT-related proteins E-cadherin, N-cadherin and Snail were detected by western blot analysis. The correlation coefficients were calculated by Pearson’s or Spearman’s test.