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. 2021 Jan 18;12(1):97. doi: 10.1038/s41419-020-03340-w

Fig. 5. MEDAG regulates breast cancer progression and EMT via AKT signaling.

Fig. 5

A KEGG enrichment analysis of DEGs of MEDAG in breast cancer. B, C Western blot analysis of p-AKT, AKT, p-AMPK, AMPK, p-mTOR, and mTOR expression in the scramble group, MEDAGKD cells, MEDAGOE cells, and NC-FLAG group in MCF-7 and MDA-MB-468. D, H p-AKT, AKT, P-AMPK, AMPK, P-mTOR, mTOR and EMT-related proteins were detected in MEDAGKD cells and MEDAGKD cells treated with SC79 (10 µg/ml, 2 h) or MEDAGOE cells and MEDAGOE cells treated with MK2206 (2 µM, 18 h) by western blot analysis. E, I Wound-healing was used to examine the migration ability in the two cell lines treated as described above. F, J Cell growth was measured by CCK-8 in breast cancer cells treated as described above. G, K Transwell assay showing the cell invasion abilities in the groups treated as described above. Right: quantitative analysis of the invasion ratio is shown. The values represent mean ± SD of three independent experiments. **P < 0.01 vs the corresponding group.