Fig. 6. MEDAG regulates breast cancer progression and EMT via the AKT/AMPK/mTOR pathway.
A Western blot analysis of p-AMPK, AMPK, p-mTOR, mTOR, and EMT protein expression in MEDAGKD cells and MEDAGKD cells treated with Compound C (10 µM, 24 h) in MCF-7 and MDA-MB-468 cell lines. B Cell growth was measured by CCK-8 in breast cancer cells treated as described above. C Transwell assay showing the cell invasion abilities in the groups treated as described above. Right: quantitative analysis of the invasion ratio is shown. D Wound-healing was used to examine the migration ability in two cell lines treated as described above. The values represent mean ± SD of three independent experiments. *P < 0.5, **P < 0.01 vs the corresponding group.