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. 2021 Jan 18;12(1):97. doi: 10.1038/s41419-020-03340-w

Fig. 8. MEDAG regulates epirubicin-induced apoptosis through the AKT signaling.

Fig. 8

A Cells were transfected with scramble siRNA or MEDAG siRNA and then treated with epirubicin alone or with SC79 for 2h before the epirubicin treatment. Cell viability was assessed by CCK-8 in cells treated as described above. B Analysis of apoptosis with FACS in MDA-MB-468 cells treated as described above. Down: quantitative analysis of the apoptosis ratio. C The expression of c-PARP was detected by western blot analysis of breast cancer cells treated as described above. Down: quantitative analysis of the optical density ratio of c-PARP compared with β-actin is shown. D–F Cells were transfected with scramble siRNA or MEDAG siRNA and then treated with epirubicin alone or with Compound C for 24h before the epirubicin treatment. Cell viability was assessed by CCK-8 and analysis of apoptosis with FACS in MDA-MB-468 cells, and the expression of c-PARP was detected by western blot analysis of breast cancer cells treated as described above. Quantitative analyses of the apoptosis ratio and the optical density ratio of c-PARP compared with β-actin are shown. The values represent mean±SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 vs the corresponding group. G Proposed model of MEDAG-induced biological function in breast cancer cells.