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. 2021 Jan 18;7:16. doi: 10.1038/s41420-020-00393-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Cyclin D1 and ki67 immunoexpression (brown staining) at E13.5+24h and E16.5+24h, respectively. Extensive, nuclear immunolabelling of the epithelial buds in the DMSO-treated buds, with sparse expression of both markers in the explants treated with Barasertib. Counting the percentage of immunopositive nuclei revealed nearly 50% loss in number of cycling (Cyclin D1) and proliferating cells (ki67), analysed by paired Student’s t test (**P ≤ 0.01, ****P ≤ 0.0001), scale bar = 20 µm.