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. 2021 Jan 18;7:16. doi: 10.1038/s41420-020-00393-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Cleaved caspase 3 (brown) immunolabelling bud cells undergoing apoptosis as a final fate following AURKB blockade for 24 h during early (E13.5+24h) and late (E16.5+24h) development, scale bar = 10 µm. B, C X-Gal assay performed on the SMG explants 24 h post DMSO or Barasertib treatments, shows significant accumulation of SA-βgal in the glands treated with the AURKB inhibitor (dotted outlines), analysed by Student’s t test (*P ≤ 0.05, n = 3 pairs). D Immunolabelling with p21 (brown staining; red arrows), 24 h post AURKB loss during early (E13.5+24h) and late (E16.5+24h) embryonic development. E Fold change of senescence-related genes: p21 was transcriptionally upregulated in both time points tested, p53 and p16 were only upregulated in the mature explants (E16.5+24h). Number of pooled glands ≥ 9 in E13.5+24h group and ≥3 in E16.5+24h obtained from three different experiments for each group. Gene expression was normalised to Gapdh and to corresponding experiment control groups and analysed by unpaired Student’s t test (*P ≤ 0.05, **P ≤ 0.01).