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. 2021 Jan 18;11:1716. doi: 10.1038/s41598-021-81173-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overview of electrode placement and timeline for the two experiments performed. (a) Electrode placement and timeline for Experiment 1. The acute effects of ethanol and flunitrazepam on GABA_A receptor mediated PSCs were measured in layer V pyramidal neurons and INs of the RSC. To electrically evoke GABA_A-PSCs, a bipolar stimulating electrode was placed on the border of layers I/II. (b) Electrode placement, laser stimulation location, and timeline for Experiment 2. The long-term effects of P7 ethanol vapor chamber exposure were measured at P40–60. PV-INs expressing channel rhodopsin-2/tdTomato were stimulated with a 473 nm laser using a 40 × objective lens in layer V of the RSC. Optically evoked IPSCs were recorded in layer V pyramidal neurons. Atlas images were adapted from the Allen Brain Coronal Atlas (©2015 Allen Institute for Brain Science. Allen Brain Atlas API. Available from: htttp://brain-map.org/api/index.html)⁸⁰. Image credit: Allen Institute.