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. 2021 Jan 18;11:1716. doi: 10.1038/s41598-021-81173-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative immunohistochemistry (IHC) images from B6 PV^cre-Ai27D mice and analysis of both penetrance and specificity of transgene expression. (a–d) Representative IHC images showing colocalization of PV and ChR2- tdTomato. (a) IHC demonstrating PV expression. (b) Expression of endogenous ChR2–tdTomato transgene expression. (c) DAPI nuclear stain. (d) Merged image. Scale bar 25 µm. (e) Penetrance analysis of B6 PV^cre-Ai27D transgene expression. Penetrance of transgene expression was measured as the number of PV expressing cells colocalized with tdTomato divided by the number of PV expressing cells. (f) Specificity analysis of B6 PV^cre-Ai27D transgene expression. Specificity of transgene expression was measured as the number of cells expressing only tdTomato divided by the number of cells expressing both PV and tdTomato. Air male n = 3 animals from 3 litters, air female n = 2 animals from 2 litters; ethanol male n = 3 animals from 3 litters, ethanol female n = 2 animals from 2 litters. Data are presented as mean ± SEM.