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. 2021 Jan 18;11:1727. doi: 10.1038/s41598-020-80826-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of average alpha-diversity scores between two different 16S sequencing methods. Box plots show the average alpha-diversity scores of both sequencing methods, which were measured by using (a) Observed_OTUs, (b) Chao 1, (c) Shannon, (d) Simpson, and (e) Pielou_e indices. Estimation of ASV richness and evenness using the number of observed OTUs (a) (Kruskal–Wallis H-test, H = 1.08, P = 0.29), Chao 1 (H = 0.0001, P = 0.99), Shannon diversity index (H = 27.43, P = 1.62*10^–7), Simpson (H = 17.17, P = 3.4*10^–5), and Pielou_e (H = 28.74, P = 8.27*10^–8) are shown. The red and blue boxes indicate average alpha-diversity of V3V4 and sFL16S, respectively. The asterisk (*) represents the p-value of the statistical test (*** < 0.0001). Detailed information for this result is in Table 2.