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. 2021 Jan 18;11:1727. doi: 10.1038/s41598-020-80826-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of bacterial taxonomy classification according to two different 16S amplicon regions. (a) The Venn-diagram is divided into two different methods (red: V3-V4 amplicon and blue: sFL16S) and shows the number of a classified bacterial taxon at the phylum to species level. (b) The heatmap shows the frequency of the only identified taxa in both methods drawn by each taxonomic rank, in order. The bacterial taxon belonging to the four major phyla (Bacteroidetes, Firmicutes, Actinobacteria, and Proteobacteria), to the species, showing the confidence score of each taxon. The darkness of the color is proportional to the confidence score's value, as shown in the color bar.