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. 2021 Jan 18;7:14. doi: 10.1038/s41420-021-00403-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A SMMC-7721 and HepG2 cells transfected with overexpressed MLXIPL plasmids or MLXIPL siRNAs were cultured for 24 h for glucose uptake assays. *P < 0.05; **P < 0.01. B Analysis of the production of lactate in SMMC-7721 and HepG2 after transfected with overexpressed MLXIPL plasmids or MLXIPL siRNAs. *P < 0.05. C The transfected cells were lysed and the cytoplasmic levels of pyruvate were detected. *P < 0.05; **P < 0.01. D The overall glycolytic flux of transfected SMMC-7721 and HepG2 cells was analyzed by ECAR using seahorse instrument. E, F Quantitative real-time PCR and western blot were performed to analyze the levels changes of glycolytic key enzymes, when transfected with overexpressed MLXIPL plasmids in SMMC-7721 and HepG2 cells. ns P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.