Table 2.

Effects of PIXI analysis on the variation between PBMC donors.

	Basal OCR		Basal GR
	mean ± s.d. (pmol/min)	CV (%)	mean ± s.d. (mpH/min)	CV (%)
Standard analysis	28.96 ± 5.11	17.7	6.38 ± 1.20	18.8
PIXI analysis	26.69 ± 3.74	14.0	5.94 ± 1.11	18.7
Effect of PIXI analysis	− 2.27 ± − 1.37	− 3.7	− 0.44 ± − 0.09	− 0.1

PBMCs were isolated from sixteen healthy lean individuals (females, 18–25 years of age, BMI 18.5–25 kg/m²) and plated at 75–300 × 10³ cells/well (n = 3–4) to generate a calibration curve that was used to transform total pixel intensity values into PIXI analyzed cell numbers. XF assay measurements were performed at 225 × 10³ cells/well (n = 8) and basal OCR and basal GR levels were normalized against the number of plated cells (standard analysis) or PIXI analyzed cells (PIXI analysis). Here a summary of the changes in group mean, s.d. and CV for basal OCR and basal GR after standard analysis or PIXI analysis is represented.