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. 2021 Jan 18;11:1665. doi: 10.1038/s41598-021-81113-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of HtrA3 were transfected in HK2 cells. (A) Real-time PCR data show HtrA3 mRNA increased after HtrA3 were transfected in ischemia/reperfusion-induced HK2 cells; (B) Western blot data show HtrA3 protein level increased after HtrA3 were transfected in ischemia/reperfusion-induced HK2 cells. N (the replicate for above experiments) = 3. *P < 0.05, **P < 0.01, ***P < 0.001, versus control. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, versus hypoxia/reoxygenation treatment group.