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. 2020 Dec 31;24(1):102022. doi: 10.1016/j.isci.2020.102022

Figure 1.

Figure 1

Reconstitution and characterization of HSPD1 oligomers

(A) SDS PAGE gel for purified HSPD1 monomer.

(B) Size exclusion chromatography of purified (gray dashed line) and reconstituted (black solid line) HSPD1 with the peak for oligomeric HSPD1 highlighted in yellow.

(C) Negative stain electron micrograph of the oligomeric HSPD1 (scale bar, 100 nm). The inset shows two representative 2D classes.

(D) Native mass spectrum of reconstituted HSPD1 showing multiple charge state distributions; different species are labeled. Above 12,000 m/z, the spectral intensity has been multiplied by 20.