Table 1.
Summary of data collection/processing parameters and model building statistics. FSC stands for Fourier Shell Correlation.
| Data collection and processing | HSPD1 single ring |
|---|---|
| Magnification | ×75,000 |
| Voltage (kV) | 300 |
| Electron exposure (e–/Å2) | 38.5 |
| Defocus range (μm) | 1.5 to 3 |
| Pixel size (Å) | 1.065 |
| Symmetry imposed | C7 |
| Initial particle images (no.) | 355,766 |
| Final particle images (no.) | 124,784 |
| Map resolution (Å) | 3.5 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.2–4.8 |
| Refinement | |
| Initial models used | PDB: 4pj1, 1xck |
| Map resolution (Å) | 3.4 |
| FSC threshold | 0.5 |
| Map sharpening B factor (Å2) | −100 |
| Model composition | |
| Non-hydrogen atoms | 27,391 |
| Protein residues | 3,675 |
| B factors (Å2) | |
| Protein | 123 |
| Root mean square deviations | |
| Bond lengths (Å) | 0.004 |
| Bond angles (°) | 0.611 |
| Validation | |
| MolProbity score | 1.74 |
| Clashscore | 10.53 |
| Poor rotamers (%) | 0.71 |
| Ramachandran plot | |
| Favored (%) | 96.75 |
| Allowed (%) | 3.25 |
| Disallowed (%) | 0 |
FSC stands for Fourier Shell Correlation.