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Chemical composition of SBF [15].
| Order | Reagent | Amount (g/L) |
|---|---|---|
| 1. | NaCl | 7.996 |
| 2. | NaHCO3 | 0.350 |
| 3. | KCl | 0.224 |
| 4. | K2HPO4.3H2O | 0.228 |
| 5. | MgCl2.6H2O | 0.305 |
| 6. | 1 mol/L HCl | 40cm3 |
| 7. | CaCl2 | 0.278 |
| 8. | Na2SO4 | 0.071 |
| 9. | (CH2OH)3CNH2 | 6.057 |
| 10. | 1 mol/L HCl | Adjust pH by dropping |
