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. 2020 Dec 24;28(1):dsaa028. doi: 10.1093/dnares/dsaa028

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© The Author(s) 2020. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Phylogenetic tree of Hao-like proteins. KSU1 C0486, which was identified as Hao5B by MBGD 27, is not shown in this figure because of its short partial sequence. Hao proteins from AOB were also included in this figure as the reference proteins. BPIT: Ca. B. pituitae, BROSI: Ca. B. sinica JPN1, EX330: Ca. Brocadia sp. BROELEC01, B6D34; Ca. Brocadia sp. UTAMX1, B6D35: Ca. Brocadia sp. UTAMX2, KSU1: Ca. J. caeni KSU1, KSMBR1: Ca. K. stuttgartiensis MBR1, SCALIN: Ca. Scalindua japonica husup-a2, Noc: Nitrosococcus oceani ATCC19707, NE: Nitrosomonas europaea ATCC 19718, Nmul: Nitrosospira multiformis ATCC 25196. Amino acid sequences of hao-like gene products were aligned by MUSCLE. Phylorgenetic relationships were inferred by Maximum Likelihood method with LG+G + I model using MEGA6.06 24.