Table 5. PCR program for library amplification.
| Step | Temp | Duration | Cycles |
|---|---|---|---|
| Initial denaturation | 98°C | 30 sec | 1 |
| Denaturation | 98°C | 10 sec | 20 |
| Annealing | 63°C | 30 sec | |
| Extension | 72°C | 1 min | |
| HOLD | 12°C | ∞ | 1 |
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