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. 2021 Jan 5;7:627848. doi: 10.3389/fmolb.2020.627848

Figure 2.

Figure 2

Dose-dependent luciferase activity induced by light in the cancer cell. (A,C) Relative luciferase activity examined of full-length dCas9 group, split dCas9 group and the negative control under dark and light condition in 293T (A) and 5637 (C) cells. (B,D) The relative luciferase activity increases with increasing light dose in 293T (B) and 5637 (D) [1s:20s-1s:120s represents 1 s illumination (0.84 W/m2) every 20–120 s]. (E) The EYFP reporter assay was used to detect changes in the expression of EYFP over time in 5,637 cells under light, dark, and light-dark conditions. (F) Time course of EYFP gene expression under light, dark, and light-dark conditions in 5,637 cell using fluorescence microscope. Data are means ± SD. (n = 3, *p < 0.05, **p < 0.01, ***p < 0.001).