Table 1.

Multiple reaction monitoring parameters for steroid analysis

Steroid metabolite	RT, min	Molecular ion, m/z	CV, V	Quantifier ion, m/z	CE, eV	Qualifier ion, m/z	CE, eV
A4	2.02	287.2	30	96.9	15	108.8	15
T	2.19	289.2	30	97.2	22	109	22
11OHA4	1.50	303.2	30	267.2	15	121	30
11KA4	1.22	301.2	35	257	25	265.2	25
11OHT	1.64	305.2	35	269	15	121	20
11KT	1.38	303.2	30	121	20	267	18
E1	3.53	504	20	171	35	156	45
E2	3.62	506	35	171	35	156	40
11OHE1	3.00	520.2	15	171	35	170	35
11KE1	2.98	518.2	15	171	35	170	35
11OHE2	3.16	522.2	15	171	35	504.2	20
11KE2	3.06	520.2	15	171	35	170	35
D2-T	2.19	291	30	99.1	20	111.2	30
D7-11OHA4	1.49	310.2	25	147.2	25	99.8	30
BPA	4.40	695	50	171	35	170	35

Retention time, molecular ion species, MRM mass transitions, CV, and CE for each steroid are reported. Internal standards: D2-T, D7-11OHA4, and BPA.

Abbreviations: 11KA4, 11-ketoandrostenedione; 11KE₁, 11-ketoestrone; 11KE₂, 11-keto-17β-estradiol; 11KT, 11-ketotestosterone; 11OHA4, 11β-hydroxyandrostenedione; 11OHE₁, 11β-hydroxyestrone; 11OHE₂, 11β-hydroxy-17β-estradiol; 11OHT, 11β-hydroxytestosterone; A4, androstenedione; BPA, bisphenol A; CE, collision energy; CV, cone voltage; D2-T, testosterone 1,2-D2; D7-11OHA4, 11β-hydroxyandrostenedione 2,2,4,6,6,16,16-D7; E₁, estrone; E₂, 17β-estradiol; eV, electron volt; MRM, multiple reaction monitoring; m/z, mass-to-charge ratio; RT, retention time; T, testosterone.