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. 2020 Dec 26;162(3):bqaa238. doi: 10.1210/endocr/bqaa238

Figure 1.

Figure 1.

miR-320a directly targets and controls the glucagon 3′ UTR. Alignment of the miR-320a seed sequence (arrow) with (A) 2 putative wild-type human glucagon 3′ UTR target sequences (hGCG-WT, bold) and the mutant target sequences (hGCG-M1 and -M2, red) and with (B) the putative wild-type mouse and rat glucagon 3′ UTR target sequences (mGcg-WT and rGcg-WT, bold) and mutated target sequences (mGcg-M and rGcg-M, blue). miR-320a–mediated effects on (C) human, (D) mouse, and (E) rat glucagon 3′ UTR luciferase reporter activity as assessed 48 hours after cotransfecting HEK293 cells with miR-320a mimic and the wild-type or mutant reporter plasmids. All data are shown as the mean ± standard error of the mean of at least 3 independent experiments, *P < 0.05. The triangles and circles represent individual measurements. N.S., not significant.