Figure 2.

miR-320a regulates endogenous glucagon mRNA levels and protein content. Mouse αTC1-6 cells were transfected with miR-320a mimic or negative control for 72 hours. (A) Effective miR-320a overexpression was confirmed and (B) glucagon mRNA expression was assessed by qRT-PCR and (C) glucagon content determined by ELISA after acid–ethanol extraction. Effects of transfection with a miR-320a inhibitor (anti-miR-320a) or a negative control on (D) miR-320a knockdown, (E) glucagon mRNA expression and (F) glucagon protein content. All data are shown as the mean ± standard error of the mean of at least 3 independent experiments, *P < 0.05. The triangles and circles represent individual measurements.