Table 2.

Primers used in the study

Primers	Sequences (5′-3′)
18s qPCR 5′ primer	GCTTAATTTGACTCAACACGGGA
18s qPCR 3′ primer	GCTATCAATCTGTCAATCCTGTCC
Human GCG 5′ primer	TGGCAACGTTCCCTTCAAGA
Human GCG 3′ primer	GATCACTGAGTGGGTCTGCC
Mouse Gcg 5′ primer	ATCTTGCCACCAGGGACTTC
Mouse Gcg 3′ primer	AAGTGACTGGCACGAGATGT
Mouse MafB 5′ primer	CCTACAAGGTCAAGTGCGAGAA
Mouse MafB 3′ primer	AGCCCGCCTCCCTGAA
Mouse Pax6 5′ primer	CCACTTCAACAGGACTCATTTCAC
Mouse Pax6 3′ primer	CACATGCTCTCTCCTTCTCTCTTTA
Mouse FoxA2 5′ primer	GGAGCCGTGAAGATGGAAG
Mouse FoxA2 3′ primer	TGTGTTCATGCCATTCATCC
Human GCG WT 3′UTR 5′ cloning primer-1	ATAACTAGTAAGAGTCCAAAGGATGGAGGGA
Human GCG WT 3′UTR 3′ cloning primer-1	ATAGTTTAAACAGAAAACAGGGGTGCTTGGA
Human GCG WT 3′UTR 5′ cloning primer-2	ATAACTAGTCTATATCACTATTCAAGATC
Human GCG WT 3′UTR 3′ cloning primer-2	ATAGTTTAAACAGACAATGGTAAAGAAGCTTGAA
Human GCG M1 3′UTR 5′ cloning primer	ATATTGATCTGGAAATATGAAAGTGC
Human GCG M1 3′UTR 3′ cloning primer	ATTTCCAGATCAATATTTTAGCAATATTTTG
Human GCG M2 3′UTR 5′ cloning primer	TACTTGATCTGCAATGTATCAAAGATAC
Human GCG M2 3′UTR 3′ cloning primer	ATTGCAGATCAAGTATTTTTAAGACAAAG
Mouse Gcg WT 3′UTR 5′ cloning primer-1/2	ATAACTAGTGTATTTCACCATTCACAACCATCTT
Mouse Gcg WT 3′UTR 3′ cloning primer-1	ATAGTTTAAACTATGGCCACCAGCTGAGAAA
Mouse Gcg WT 3′UTR 3′ cloning primer-2	ATAGTTTAAACTTTATTTAAATCTATTCTTGATACATC
Mouse Gcg M 3′UTR 5′ cloning primer	CACTTGATCTGGTGATGTATCAAGAATAG
Mouse Gcg M 3′UTR 3′ cloning primer	ATCACCAGATCAAGTGCATTTATGGTAAAG
Rat Gcg WT 3′UTR 5′ cloning primer-1	ATAACTAGTAACAATTCCTGCTTTTGTCCTCA
Rat Gcg WT 3′UTR 3′ cloning primer-1	ATAGTTTAAACATGGTAGAGTACTGCACGGC
Rat Gcg WT 3′UTR 5′ cloning primer-2	ATAACTAGTGAATATTTCACCATTCACAACC
Rat Gcg WT 3′UTR 3′ cloning primer-2	ATAGTTTAAACTAATAACAAAGGATGAGATATTTA
Rat Gcg M 3′UTR 5′ cloning primer	CGCTTGATCTGGCAATGTATCAAGAATAG
Rat Gcg M 3′UTR 3′ cloning primer	ATTGCCAGATCAAGCGCATTTATGACAAAG