Figure 6.

Melanization in Galleria mellonella larvae infected with strains of B. cenocepacia occurs in the presence of the pathogen and does not depend on the presence of OLs. (A) Control larvae infiltrated with 10 mM MgSO₄, (B) Larvae infected with B. cenocepacia wild-type J2315, and (C) larvae infected with the B. cenocepacia mutant strain NG1. The pathogenicity assays were carried out using 10 insect larvae placed individually in 55 mm Petri dishes without diet and incubated at 30°C, evaluating mortality every 24 h after injection for 5 days before moving to the next larval growth stage. The melanization in G. mellonella larvae occurred along with larva death. Five independent experiments were performed.