Figure 8.

The presence of ornithine lipids in R. andropogonis drastically increases the formation of reactive oxygen species (ROS) directly after infiltration into leaves of maize plants. ROS detection as DCFH-DA fluorescence (A) in maize leaves treated with mock solution, (B) treated with LMG2129.pRK404.pET9a, or (C) treated with LMG2129.pRK404.pET9a.olsF. (D) Densitometric quantification of ROS production by measuring DCFH-DA fluorescence showed that immediately after infection (0 dpi) the ROS levels were two times higher in leaves infiltrated with the strain constitutively forming OLs compared to leaves infiltrated with the wild-type strain, and were significantly different (*) determined by the analysis tailed t-test with a P-value: 0,0263 at 0 dpi. ROS concentration was lower on later times at 3, 7, and 15 dpi, and no differences were detected between the treatments. The experiment was repeated three times with similar results.