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. 2021 Jan 5;7:610932. doi: 10.3389/fmolb.2020.610932

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Copyright © 2021 Córdoba-Castro, Salgado-Morales, Torres, Martínez-Aguilar, Lozano, Vences-Guzmán, Guan, Dantán-González, Serrano and Sohlenkamp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The presence of OLs does not modify the progression of the infection of R. andropogonis in maize leaves. When infiltrated with R. andropogonis strains, maize leaves showed dark-red lesions and chlorosis, while control plants infiltrated with the mock solution were asymptomatic. (A) Mock solution, (B) wild-type LMG2129, (C) LMG2129.pRK404.pET9a, and (D) LMG2129.pRK404.pET9a.olsF. (E) The number of bacteria isolated from the maize leaves at 0, 3, 7, and 15 dpi, increased over time in infected plants, and no significant differences could be observed between the strains. The experiment was repeated three times with similar results using thirty corn plants in total for each treatment.