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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Drug Alcohol Depend. 2020 Sep 21;216:108309. doi: 10.1016/j.drugalcdep.2020.108309

Figure 1. Increased excitability of layer 5 pyramidal neurons in the PrL 21 days, but not 2 days, after Ado::CIE procedure.

Figure 1.

A, Detailed experimental time line.

B, Location of whole-cell patch clamp recordings in PrL-L5.

C, Example traces of action potentials elicited by 250 and 500 pA current injections to a PrL-L5 pyramidal neuron 2 days after Ado::CIW and Ado::CIE.

D, Summarized data showing no changes of spike number recorded from PrL-L5 pyramidal neurons 2 days after the Ado::CIW vs. Ado::CIE (Iinj × CIW/CIE interaction F10,320=0.4, p=0.92, CIW/CIE F1,32=0.4, p=0.55, cell-based; Iinj × CIW/CIE interaction F10,180=0.3 p=0.98, CIW/CIE F1,18=0.4, p=0.53, animal-based).

E, Example traces of action potentials elicited by 250 and 500 pA current injections to a PrL-L5 pyramidal neuron 21 days after Ado::CIW and Ado::CIE.

F, Summarized data showing an increased excitability of PrL-L5 pyramidal neurons 21 days after Ado::CIE (Iinj × CIW/CIE interaction F10,310=2.6, p<0.01, CIW/CIE F1,31=4.9, p=0.03, cell-based; Iinj × CIW/CIE interaction F10,130=3.1, p<0.01, CIW/CIE F1,13=5.1, p=0.04, animal-based).

Data were analyzed by two-way ANOVA with repeated measures (D, F), followed by Bonferroni post-hoc tests. n/m, the number of cells/animals for data collection. *, p<0.05, CIW vs. CIE at the specific injection current.