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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Drug Alcohol Depend. 2020 Sep 21;216:108309. doi: 10.1016/j.drugalcdep.2020.108309

Figure 2. Decreased excitability of layer 5 pyramidal neurons in the PrL 21 days, but not 2 days, after Adu::CIE.

Figure 2.

A, Experimental time line, see more details in Fig. 1A.

B, Location of whole-cell patch clamp recordings in PrL-L5.

C, Example traces of action potentials elicited by 250 and 500 pA current injections to a PrL-L5 pyramidal neuron 2 days after Adu::CIW and Adu::CIE.

D, Summarized data showing no changes of spike number recorded from PrL-L5 pyramidal neurons 2 days after the Adu::CIW vs. Adu::CIE (Iinj × CIW/CIE interaction F10,200=0.1, p=0.99, CIW/CIE F1,20=0.3, p=0.59, cell -based; Iinj × CIW/CIE interaction F10,90=0.5, p=0.90, CIW/CIE F1,9=0.2, p=0.66, animal -based).

E, Example traces of action potentials elicited by 250 and 500 pA current injections to a PrL-L5 pyramidal neuron 21 days after Adu::CIW and Adu::CIE.

F, Summarized data showing a decreased excitability of PrL-L5 pyramidal neurons 21 days after Adu::CIE (Iinj × CIW/CIE interaction F10,310=2.2, p=0.02, CIW/CIE F1,31=3.4, p=0.07, cell -based; Iinj × CIW/CIE interaction F10,120=2.0, p=0.03, CIW/CIE F1,12=3.1, p=0.10, animal -based).

Data were analyzed by two-way ANOVA with repeated measures (D, F), followed by Bonferroni post-hoc tests. n/m, the number of cells/animals for data collection. *, p<0.05, CIW vs. CIE at the specific current injection.