Figure 3. Effects of CIE on sEPSCs recorded from PrL-L5 pyramidal neurons.

A, Representative traces of sEPSCs 2 days after adolescent CIW/CIE treatment and early-adult CIW/CIE treatment.

B, Summarized data showing no effects of adolescent or early-adult CIE on the frequency of sEPSCs (CIW/CIE × Ado/Adu interaction F_1,48=0.1, p=0.72, CIW/CIE F_1,48=0.6, p=0.46, Ado/Adu F_1,48<0.1, p=0.87, cell-based; CIW/CIE × Ado/Adu interaction F_1,22=0.3, p=0.61, CIW/CIE F_1,22=0.1, p=0.71, Ado/Adu F_1,22=0.1, p=0.72, animal-based).

C, Summarized data showing significant decrease of sEPSC amplitude 2 days after adolescent, but not early-adult, CIE (CIW/CIE × Ado/Adu interaction F_1,48=8.3, p<0.01, CIW/CIE F_1,48=11.5, p<0.01, Ado/Adu F_1,48=1.0, p=0.32, cell-based; CIW/CIE × Ado/Adu interaction F_1,22=8.4, p=0.01, CIW/CIE F_1,22=11.7, p<0.01, Ado/Adu F_1,22=1.2, p=0.29, animal-based).

D, Representative traces of sEPSCs 21 days after adolescent CIW/CIE treatment and early-adult CIW/CIE treatment.

E, Summarized data showing that sEPSC frequency significantly decreased and increased 2 days after adolescent and early-adult CIE, respectively (CIW/CIE × Ado/Adu interaction F_1,62=4.4, p=0.04, CIW/CIE F_1,62=0.1, p=0.79, Ado/Adu F_1,62=3.9, p=0.05, cell-based; CIW/CIE × Ado/Adu interaction F_1,25=7.8, p=0.01, CIW/CIE F_1,25<0.1, p=0.85, Ado/Adu F_1,25=6.5, p=0.02, animal-based).

F, Summarized data showing that sEPSC amplitude significantly decreased and increased 2 days after adolescent and early-adult CIE, respectively (CIW/CIE × Ado/Adu interaction F_1,62=108.3, p<0.01, CIW/CIE F_1,62=4.4, p=0.04, Ado/Adu F_1,62=99.8, p<0.01, cell-based; CIW/CIE × Ado/Adu interaction F_1,25=116.5, p<0.01, CIW/CIE F_1,25=4.7, p=0.04, Ado/Adu F_1,25=102.8, p<0.01, animal-based).

Data were analyzed by two-way ANOVA (B,C,E,F), followed by Bonferroni post-hoc tests. n/m, the number of cells/animals.