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. 2021 Jan 19;220(3):e201908108. doi: 10.1083/jcb.201908108

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© 2021 Allam et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 4. — Notch and CXCR4 signaling regulate the pre-TCR downstream signaling and are required for progression beyond β-selection. (A) DN3a cells were cocultured with OP9-DL1 cells in the presence or absence of either Notch inhibitor (DBZ) or CXCR4 inhibitor (AMD3100). After 5 d, flow cytometry was used to assess the effect on Notch and CXCR4 inhibition on DN3a cell progression and differentiation. The effect of either Notch or CXCR4 inhibition on survival was assessed using PI intensities (as shown by bottom row histograms). (B) To assess the effect of Notch or CXCR4 inhibition on pre-TCR downstream signaling, purified DN3a cocultures were treated with either Notch inhibitor (DBZ) or CXCR4 inhibitor for 3 h and then fixed, and levels of pLCK394 were assessed as a marker of pre-TCR downstream signaling (as shown by histograms). Hes1 and CXCR4 (top row) were stained to assess the effectiveness of inhibition of Notch and CXCR4, respectively. The results are representative of three independent biological replicates.