Video 1.
F-actin relocates to the front of DN3a cells upon docking onto OP-DL1. Time-lapse confocal microscopy showing DN3a cell stained with SiR-Actin (red, top right frame) cocultured with OP-DL1 cells (green, top left). Top left: SiR-Actin polarizes and stabilizes at the interface between DN3 and OP-DL1 cells (green, top right frame) for a period of ∼54 min (n = 22). Bottom left: DIC of field of view; bottom right: merge of the SiR-Actin and DIC frames. Each frame is 3 min, and playback is 3 frames per second.