Figure S1.

Further characterization of the effect of starvation on the AP-5/SPG11/SPG15 complex. (A) IF double labeling of SPG11-GFP (amplified with anti-GFP) and either EEA1 as a marker of early endosomes or LAMP1 as a marker of late endosomes/lysosomes, under either fed or starved conditions (1 h). Scale bar: 20 µm. (B) HeLa cells were transiently transfected with HA-SPG15 and double labeled for HA and either EEA1 or LAMP1, under either fed or starved conditions (1 h). Note the starvation-induced enhanced recruitment of both SPG11-GFP and HA-SPG15 to LAMP1-positive late endosomes/lysosomes. Scale bar: 20 µm. (C) Localization of SPG15-GFP (amplified with anti-GFP) under control conditions or starved 1 h, using the indicated conditions. dFCS, dialysed FCS. Scale bar: 20 µm.