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. 2021 Jan 19;220(2):e202002075. doi: 10.1083/jcb.202002075

Figure S2.

Figure S2.

Effects of PI3K and PI5K inhibition on different components of the AP-5/SPG11/SPG15 complex. (A) Localization of SPG11-GFP (amplified with anti-GFP) under control conditions or after treatment with either a PI3K inhibitor (100 nM wortmannin; 1 h) or a PI5K inhibitor (1 µM YM201636; 1 h). Like SPG15-GFP, SPG11-GFP is lost from the membrane upon PI3K inhibition, but membrane localization is enhanced upon PI5K inhibition. Scale bar: 20 µm. (B) IF double labeling of HA-tagged SPG15 and LAMP, either under control (fed) conditions or after treatment with a PI5K inhibitor (1 µM YM201636; 1 h). Membrane localization of the HA-tagged SPG15 is much more apparent after PI5K inhibition. Scale bar: 20 µm. (C) IF double labeling of AP5Z1-GFP (amplified with anti-GFP) and LAMP, either under control (fed) conditions or after treatment with a PI5K inhibitor (1 µM YM201636; 1 h). Membrane localization of AP5Z1-GFP is normally difficult to discern, but it is enhanced by PI5K inhibition. Scale bar: 20 µm.