Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 18;18:22. doi: 10.1186/s12985-020-01484-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Autophagy is involved in H9N2 influenza virus replication (II). A549 cells were pre-treated with LY294002, control siRNA or Atg5 siRNA, followed by infection with the H9N2 influenza virus. The control siRNA is the scramble siRNA and it has the same nucleotide composition as the target gene sequence. (a) The protein expression levels of Atg5 were detected by western blot analysis. (b, d) Cells were collected and the protein expression levels of Atg5, LC3-II, NP, and M2 were detected by western blot analysis; **P < 0.01 relative to control, ^##P < 0.01 relative to H9N2. (c) Supernatants from A549 cells were collected, and the viral titer was determined by measuring TCID₅₀;**P < 0.01 relative to H9N2. (e, f) NP mRNA was detected by RT-PCR;**P < 0.01 relative to H9N2. The data are presented as the means ± SD (n = 5)