Fig. 2.

Stereotyped behavior in Shank3 mice. a–d Grooming time and frequency in Shank3 mice. Mice were placed in a clean cylinder and videotaped. a–c There was no difference in the time spent grooming within all groups of mice. b–d However, compared to WT, grooming frequency was increased in Shank3 ΔC/ΔC and Shank3+/ΔC males. Only Shank3 ΔC/ΔC females showed increased grooming compared to WT and Shank3+/ΔC (B). All data are expressed as means ± SEM. One-way ANOVA followed by Tukey’s multiple analysis was performed (*p < 0.05, **p < 0.01, ***p < 0.001) WT males n = 20; Shank3+/ΔC males n = 12; Shank3 ΔC/ΔC males, n = 7; WT females n = 12; Shank3+/ΔC females n = 12; Shank3 ΔC/ΔC females, n = 13