Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 19;12:70. doi: 10.1186/s13287-021-02134-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Induction of iPSC to retinal progenitors and their characterization. a TC-1133 hiPSC colony. b hiPSC expressing more than 95% OCT4. c Formation of embryoid bodies from iPSC and their induction to retinal progenitors. d–g Immunofluorescence images representing the protein expression at day 20 before selection. d Neuroectodermal markers PAX6, Nestin, SOX1, and SOX2. e Retinal commitment markers RX, LHX2, CRX, and OTX2. f Neural markers FOXG1 and GFAPΔ. g Expression of OCT4 and Ki67. h Gene quantification using real-time PCR of selected markers in day 20 retinal progenitors and day 25 RPE progenitors and photoreceptor progenitors after rosette selection represented as fold change with respect to the undifferentiated iPSC. Images and graphs are representative of a minimum of three independent experiments. Scale bars represent 100 μm