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. 2021 Jan 12;8(1):ENEURO.0328-20.2020. doi: 10.1523/ENEURO.0328-20.2020

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Copyright © 2021 Kristaponyte et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 1. — Characterization of mixed CBA/CaJ x C57BL/6J genetic background mice. A, ABR thresholds (expressed as back-transformed least squares means) were not significantly different between ChR2-EYFP-positive (ChR2-EYFP⁺) and ChR2-EYFP-negative (ChR2-EYFP^–) littermates. Error bars represent 95% confidence intervals of means. B, C, ChR2 expression in inhibitory IC central nucleus neurons. B, A low-magnification image shows that the majority of inhibitory cells labeled with anti-GAD67 antibody (red) also express ChR2-EYFP labeled with anti-GFP antibody (green). White arrows show examples of double-labeled cells. The blue arrow shows a rare example of a cell that labeled with the anti-GAD antibody but did not express EYFP. Scale bar: 20 μm. C, High-magnification images of double-labeled cells. Scale bar: 20 μm.