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. 2021 Jan 19;9(2):e14696. doi: 10.14814/phy2.14696

FIGURE 2.

FIGURE 2

Delivery of Klotho inhibits Wnt/β‐catenin signaling in UIRI mice. (a–c) Representative micrographs and quantitative graphs show exogenous Klotho suppresses the expression Wnt1 and Wnt9a in UIRI mice. Arrows indicate positive staining. Scale bar, 50 μm. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6). (d) Representative western blots show Wnt1 and Wnt9a expression in three groups, as indicated. (e and f) Graphical representations of (e) Wnt1 and (f) Wnt9a protein expression levels in different groups, as indicated. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6). (g–h) Representative immunofluorescence micrographs and quantitative data show active β‐catenin expression in three groups. Arrow indicates positive staining. Scale bar, 50 μm. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6). (i–l) Representative western blot (i) and quantitative data show (j) β‐catenin, (k) PAI‐1, and (l) MMP‐7 protein expression levels in different groups, as indicated. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6).