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. 2021 Jan 19;9(2):e14696. doi: 10.14814/phy2.14696

FIGURE 4.

FIGURE 4

Klotho inhibits tubular cell senescence in UIRI mice. (a–c) Representative staining micrographs and quantitative graph show SA–β‐gal activity and p16INK4a expression in different groups. Frozen kidney sections were stained for SA–β‐gal activity, which appears as bright‐blue granular staining in the cytoplasm of tubular epithelial cells. Paraffin sections were immunostained with an antibody against p16INK4a. Arrows indicate positive staining. Scale bar, 50 μm. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6). (d) Representative western blots show renal expression of p16INK4a, p19ARF, and γH2AX in three groups, as indicated. (e–g) Graphical representations show the expression levels of (e) p16INK4a, (f) p19ARF, and (g) γH2AX in three groups. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6). (h and i) Relative mRNA abundance of (h) p19ARF and (i) γH2AX in different groups is shown. *p < 0.05 versus sham controls; † p < 0.05 versus UIRI alone (n = 5–6).