TABLE 2.
Antibodies used in the study.
| Protein target | Name of antibody | Manufacturer (Catalog no., Lot no., RRID:) | Species raised in; monoclonal or polyclonal | Dilution, techniques & relevant references |
|---|---|---|---|---|
| VEGFA | Anti‐VEGF antibody (EP1176Y) | Abcam (Cat no. ab52917, Lot no. GR3219705‐8, RRID: AB_883427) | Rabbit polyclonal | 1:10,000 in Western blot (Wang et al., 2020) |
| VEGFA | VEGF antibody (VG1) | Novus Biologicals (Cat no. NB100‐664, Lot no. E‐4, RRID: AB_10001947) | Mouse monoclonal | 1:100 in Immunohistochemistry (O'Byrne et al., 2000; Turley et al., 1998) |
| PlGF | PlGF (H‐4) | Santa Cruz (Cat no. sc‐518003, Lot no. G0318, RRID: AB_2861376) | Mouse monoclonal | 1:1000 in Western blot (Zhao et al., 2019) |
| VEGFR1 | Anti‐VEGF receptor 1 antibody [Flt‐1/EWC] | Abcam (Cat no. ab9540, Lot no. GR3237737‐2, RRID: AB_307328) | Mouse monoclonal | 1:1000 in Western blot (Lin et al., 2020) |
| VEGFR1 | Anti‐VEGF receptor 1 antibody [Y103] | Abcam (Cat no. ab32152, Lot no. GR157619‐56, RRID: AB_778798) | Rabbit monoclonal | 1:100 in Immunohistochemistry (Astern et al., 2013) |
| VEGFR2 | Flk‐1 (A‐3) | Santa Cruz (Cat no. sc‐6251, Lot no. C1119, RRID: AB_628431) | Mouse monoclonal | 1:500 in Western blot (Bersini et al., 2018; Francis & Wei, 2010); 1:50 in Immunohistochemistry (Möller et al., 2005) |
| Actin | Monoclonal anti‐β‐actin | Sigma (Cat no. A2228, Lot no. 067M4856V, RRID: AB_476697) | Mouse monoclonal | 1:10,000 in Western blot (Mpilla et al., 2019; Palme et al., 2020) |
| Secondary | Goat anti‐mouse IgG | Bio‐Rad Laboratories (Cat no. 170‐6516, RRID: AB_11125547) | Goat | 1:10,000 in Western blot |
| Secondary | Goat anti‐mouse whole IgG affinity‐purified antibodies | Jackson ImmunoResearch Laboratories (Cat no. 115‐065‐003, Lot no. 139869, RRID: AB_2338557) | Goat | 1:500 in Immunohistochemistry |
| Secondary | Goat anti‐rabbit whole IgG affinity‐purified antibodies | Jackson ImmunoResearch Laboratories (Cat no. 111‐065‐003, Lot no. 140103, RRID: AB_2337959) | Goat | 1:500 in Immunohistochemistry |