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. Author manuscript; available in PMC: 2021 Aug 18.
Published in final edited form as: Acc Chem Res. 2020 Jul 21;53(8):1593–1604. doi: 10.1021/acs.accounts.0c00292

Figure 9.

Figure 9.

BIST-caged Ca2+ and GABA probes – chromophore construction and photochemical properties.

A. Construction of the BIST chromophore and elaboration into BIST-2EGTA and G3.5-BIST-GABA. B. Extinction coefficients of a range of o-nitrobenzyl caging chromophores. C. Comparison of the efficacy of 2P uncaging of Ca2+ from BIST-2EGTA and DM-nitrophen in a droplet. D. Structure of BIST-caged GABA. D. G3.5-BIST-GABA is inert up to 2 mM, but RuBi-GABA is highly antagonistic at 0.35 mM. E. GABA-A receptor currents (inhibitory postsynaptic currents, IPSCs) recorded from a CA1 neuron. Upper: Single sweep showing 2pIPSCs are indistinguishable from spontaneous currents. Bottom: average of 24 sweeps showing only the 2pIPSCs. F. Brief light flashes can evoke rapid IPSCs similar to spontaneous currents. D-F: Reproduced with permission from ref. 82. Copyright (2019) Wiley.