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. 2021 Jan 18;14:54. doi: 10.1186/s13071-020-04541-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Antibody response of the IgM (a) and IgG (b) subclass specific to Mesocestoides (M.) vogae homogenate (MvH) or excretory/secretory molecules (ES) in the peritoneal exudates of healthy and infected mice at 3, 6, 10, 14, 19, 25, 30 and 35 days post-infection (p.i.). The anti-M. vogae IgM and IgG antibody titers were determined by ELISA methods. Data are presented as the means of OD ± SD. OD values of IgM for uninfected mice (n = 3) were 0.059 ± 0.004 for MvH antigen and 0.067 ± 0.012 for ES. OD values of IgG for uninfected mice (n = 4) were 0.141 ± 0.025 for MvH antigen and 0.079 ± 0.013 for ES. The cut-off values are indicated in the figures. Statistical significance was analyzed using two-way ANOVA followed by Bonferroni's multiple comparison tests, and significantly different values are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001. Detection of immunogenic antigens within MvH and ES preparations immunoreactive to IgG (c MvH; d ES) and IgM antibodies (e MvH; f ES) performed by western blot analysis. Exudates from infected mice obtained on corresponding days p.i. were incubated with nitrocellulose strips with blotted MvH or ES antigens.