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. 2021 Jan 18;14:54. doi: 10.1186/s13071-020-04541-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Peritoneal inflammation during Mesocestoides (M.) vogae infection is associated with an increased number of myeloid CD11b+Gr-1+ cells. Myeloid cell population in the peritoneal cavities of healthy and M. vogae-infected mice at 3, 6, 10, 14, 19, 25, 30 and 35 days post-infection. Peritoneal exudate cells (PEC) were isolated from the peritoneal cavity, and the expression of myeloid cell surface markers by these cells was analyzed using flow cytometry (FC). Live cells were gated on CD11b and Gr-1 expression to first identify CD11b+Gr-1+ cells. Then, F4/80 and MHC II expression was identified within the CD11b+Gr-1+ cells. a Representative dot plots obtained by FC analysis show the expression of CD11b/Gr-1 and F4/80/MHC II on PEC. b Proportions of CD11b^highGr-1+ cells in the peritoneal cavities of healthy and infected mice. c Proportions of F4/80+MHC II+ cells within CD11b^highGr-1+ cells in the peritoneal exudate cell population from healthy and infected mice. F4/80 (d) and MHC II (e) mean fluorescence intensity (MFI) within CD11b^highGr-1+ cells in the peritoneal exudate cell population from healthy and infected mice. Data are expressed as the mean ± SD. Statistical significance was analyzed using one-way ANOVA with Dunnett's post-test, and significantly different values between healthy and infected mice are indicated as: *p < 0.05, **p < 0.01, ***p < 0.001.