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. 2021 Jan 18;14:54. doi: 10.1186/s13071-020-04541-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Mesocestoides vogae excretory-secretory (ES) products induce the accumulation of CD11b+IL-10+ peritoneal cells. Mice were injected intraperitoneally with 20 µg of ES and PBS as control every day for a total of 6 days. Peritoneal exudate cells (PEC) were isolated and subjected to flow cytometric analysis. a Representative flow cytometry plots showing the expression of IL-10 and CD11b by PEC. Live cells were gated on CD11b expression to first identify CD11b+ cells. Then, IL-10 expression was identified within CD11b+ cells. b Proportions of CD11b+IL-10+ cells in the peritoneal cavities of PBS and ES treated mice. c Concentrations of IL-10 in the peritoneal exudates was measured by ELISA and expressed in pg/ml. d Relative expression of m-RNA for IL-10 in the peritoneal exudate cells was analyzed by real-time PCR. Data are expressed as the mean ± SD. Statistical significance was analyzed using Student's t test, and significantly different values between healthy and ES treated mice are indicated as: *p < 0.05, **p < 0.01.